Plasmid electrotransfer into mouse soleus muscle

Abstract

Plasmid electrotransfer into mouse soleus muscle may help to assess gene effect on muscle function. The aim if this study was to evaluate transfection efficiency of superficial tibialis anterior muscle plasmid electrophoretic transfer protocol for deeper soleus muscle. Plasmid with green fluorescent protein was injected into soleus muscle and thereafter eight square-wave 20 ms and 200 V/cm electrical pulses at 1 Hz were used to induce the transfection. Transfection efficiency was ~22 % thus methodology adjustments are needed when higher level of transfection is required.