Comparison of two sampling methods for detection of Lawsonia intracellularis in industrial pig farms

Raimundas Lelesius

Abstract


The aim of this study was to compare the efficiency of two sampling methods to provide material for the detection of L. intracellularis by nested PCR. Prevalence of L. intracellularis was evaluated in diseased and dead diarrhoeic weaners and fatteners in 49 cases of outbreaks of enteric diseases. Faecal (n=262) and ileum mucosal scrapings (n=66) were tested.

Proliferative enteropathy was diagnosed in 26.5% (13/49) cases of outbreaks of enteric diseases: 26.7% (4/15) ones in weaners and 26.5% (9/34) ones in fatteners. L. intracellularis was detected in 7.2% (19/262) faecal samples and in 21.2% (14/66) ileum mucosal. The results of this study demonstrated that L. intracellularis is important pathogen in swine farms. We assure, that ileum mucosa sampling is a very suitable method for rapid L .intracellularis detection.


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BIOMEDICAL ENGINEERING CONFERENCE ORGANIZING COMMITEE,

BIOMEDICAL ENGINEERING INSTITUTE,

KAUNAS UNIVERSITY OF TECHNOLOGY.