Evaluation of the Background Extraction Influence to Quantitative Analysis of Two-Dimensional Electrophoresis Gel Images

Abstract

In our investigation, presented in this paper, we have evaluated the influence of two different background subtraction techniques to the results of quantitative analysis of same proteins emphasized in gel using different staining techniques. Using 2DE gel image background extraction the variation of the estimated protein quantity increases more than 50% for 12%–33% of protein spots and more than 100% for 4.3%–12% protein spots.