Spectrofotometrical determination of reduced glutathion and lipid peroxidation in liver of cadmium and zinc treated mice

Abstract

The present investigation was undertaken to evaluate the influence of Cd and Zn on the content of GSH and MDA. Experiments were done on outbred white laboratory mice using intraperitoneal injections of CdCl2 or ZnSO4 solution. The exposure-time was 2 h, 8 h, 24 h and 14 days. Our results showed that after 8 h the content of GSH was increased by 35% after exposure to Cd and 27% after exposure to Zn. After 14 days the content of GSH in mice liver was decreased by 32% after exposure to Cd. The content of MDA was increased after 8 h by 236% and 24 h by 118% after exposure to Cd. Moreover, 24 h after exposure to Zn, MDA content was increased by 226% as compared to the control.